Part:BBa_K2015009:Experience
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Applications of BBa_K2015009
We combined BBa_K2015012 and BBa_K2015009 to be quantified. BBa_K2015012 contains plac(BBa_R0011) and it may be induced by IPTG. If it works correctly, it expresses green fluorescence with IPTG. We added IPTG and analyzed a GFP expression level. Fig. 1. shows a result. From left, BBa_K741002 as a positive control (cntaining a GFP expression unit), BBa_K2015012-BBa_K2015009 with IPTG 0mM, 0.5mM, 1.0mM, BBa_K2015012-BBa_K2015009 from different colony with 0mM, 0.5mM, and 1.0mM. BBa_K741002 just expressed GFP and BBa_K2015012-BBa_K2015009 didn't light at all. We couldn't recognize GFP with IPTG 0mM, 0.5mM, and 1.0mM and couldn't get a positive result.
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| Fig. 1. BBa_K2015012-BBa_K2015008 induced by IPTG BBa_K2015012-BBa_K2015009 on pSB1C3 vector were induced by IPTG and incubated at 25°C for 24 h. (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG |
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Fig. 2. BBa_K2015012-BBa_K2015009 induced by IPTG BBa_K2015012-BBa_K2015009 on pSB1C3 vector were induced by IPTG and incubated at 37°C for 16 h. (-): not induced, (+): 0.5 mM IPTG and (++): 1.0 mM IPTG |
